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Farmacologia Generale E Clinica Katzung Pdf

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In fact, when the antibiotic treatment kills normal-growing cells, persisters survive and resuscitate upon treatment interruption, thus repopulating the biofilm Lewis, The physical shielding offered by the extracellular matrix, the bacterial resistance Brauner et al.

The increasing use in medicine of indwelling devices, such as orthopedic joint prosthesis, cardiac valves, breast implants and also catheters, fosters the development of infections difficult to treat due to bacteria including S.

Vancomycin remains one of the pharmacological choices for the prophylaxis or treatment of invasive staphylococcal infections Liu et al. This glycopeptide exerts a slow bactericidal activity against gram positive bacteria inhibiting the cell wall biosynthesis by strongly binding to the terminal D-Ala-D-Ala dipeptide on the nascent peptidoglycan chains Katzung et al.

The aims of this study are: i to investigate the in vitro anti-biofilm activity of selected antibiotics commonly used in the clinical practice for the management of implant-associated infections due to methicillin- resistant and methicillin-susceptible S.

Inocula were prepared according to a McFarland standard turbidity of 0. Antimicrobial Agents Fosfomycin InfectoPharm, Heppenheim, Germany , rifampicin Sandoz Pharmaceuticals, Steinhausen, Switzerland and vancomycin Hexal, Holzkirchen, Germany were obtained as powder and reconstituted using sterile pyrogen-free water.

Daptomycin Novartis Pharma Schweiz, Basel, Switzerland was obtained as a powder and dissolved in sterile pyrogen-free 0. The MIC was defined as the lowest concentration of antimicrobial that completely inhibited the growth of the organism in the tubes, as detected by unaided eye. Glass ampoules were sealed for air tightness and introduced into the microcalorimeter, first in the equilibration position and, after 15 min, in the measuring position.

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The minimum heat inhibitory concentration MHIC for planktonic was defined as the lowest antimicrobial concentration that inhibited growth-related heat production during 24 h-incubation in the microcalorimeter, as previously reported Oliva et al. For each antibiotic two independent experiments were performed, if the two MHIC values were discordant, a third experiment was carried out.

The ratio between glass beads and bacterial suspension ml was After incubation, beads were washed three times with 0.

A positive control consisting in biofilm incubated without treatment was included, as well as a sterile bead as a negative control.

The minimum biofilm bactericidal concentration MBBC was defined as the lowest antimicrobial concentration that strongly reduced the number of viable bacterial cells within the biofilm, therefore leading to undetectable heat values no biofilm cell metabolic activity during 48 h-incubation in fresh medium Butini et al.

For each antibiotic two independent experiments were performed, if the two MBBC values were discordant, a third experiment was carried out.

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The biofilm beads treated with MBBC of each antibiotic were collected and further analyzed by sonication and colony counting. Briefly, after calorimetric analysis, beads were washed and transferred to a 2 ml Eppendorf tubes containing 1 ml PBS pH 7. Beads with untreated biofilm were included as growth control, as well as sterile beads as negative control.

Next, beads were washed and sonicated as described above to dislodge cells survived to the antibiotic treatment. Please choose whether or fxrmacologia you want other users to be able to see on your profile that this library is a favorite of yours. Your rating has been recorded.

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Farmacologia Básica e Clínica - Katzung

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Paoletti, S. Antimicrobial Activity of Daptomycin Versus Persister Cells Survived After Vancomycin Treatment To evaluate a potential synergistic eradicating activity of the sequential use of vancomycin and daptomycin against staphylococcal biofilm, a 24 h-old MRSA biofilm was formed on porous glass beads, as previously reported.

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